Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants.

A cDNA for duck liver 'malic' enzyme (EC 1.1.1.40) was subcloned into pUC-8, and the active enzyme was expressed in Escherichia coli TG-2 cells as a fusion protein including a 15-residue N-terminal leader from beta-galactosidase coded by the lacZ' gene. C99S and R70Q mutants of the en...

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Detalhes bibliográficos
Main Authors: Hsu, R Y, Glynias, M J, Satterlee, J, Feeney, R, Clarke, A R, Emery, D C, Roe, B A, Wilson, R K, Goodridge, A G, Holbrook, J J
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132620/
https://ncbi.nlm.nih.gov/pubmed/1622402
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