Crystallization scale purification of α7 nicotinic acetylcholine receptor from mammalian cells using a BacMam expression system

AIM: To report our methods for expression and purification of α7 nicotinic acetylcholine receptor (α7-nAChR), a ligand-gated pentameric ion channel and an important drug target. METHODS: α7-nAChRs of 10 different species were cloned into an inducible BacMam vector with an N-terminal tag of a tandem...

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Dettagli Bibliografici
Pubblicato in:Acta Pharmacol Sin
Autori principali: Cheng, Hao, Fan, Chen, Zhang, Si-wei, Wu, Zhong-shan, Cui, Zhi-cheng, Melcher, Karsten, Zhang, Cheng-hai, Jiang, Yi, Cong, Yao, Xu, H Eric
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2015
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4564878/
https://ncbi.nlm.nih.gov/pubmed/26073323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/aps.2015.34
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