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Entropic Enhancement of Protein-DNA Affinity by Oxygen-to-Sulfur Substitution in DNA Phosphate

Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallogra...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Zandarashvili, Levani, Nguyen, Dan, Anderson, Kurtis M., White, Mark A., Gorenstein, David G., Iwahara, Junji
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4564683/
https://ncbi.nlm.nih.gov/pubmed/26331260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.07.032
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