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A chemical approach for site-specific identification of NMR signals from protein side-chain NH(3)(+) groups forming intermolecular ion pairs in protein–nucleic acid complexes
Protein–nucleic acid interactions involve intermolecular ion pairs of protein side-chain and DNA or RNA phosphate groups. Using three protein–DNA complexes, we demonstrate that site-specific oxygen-to-sulfur substitution in phosphate groups allows for identification of NMR signals from the protein s...
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| Publicado no: | J Biomol NMR |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4433575/ https://ncbi.nlm.nih.gov/pubmed/25690740 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-9909-8 |
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