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A chemical approach for site-specific identification of NMR signals from protein side-chain NH(3)(+) groups forming intermolecular ion pairs in protein–nucleic acid complexes

Protein–nucleic acid interactions involve intermolecular ion pairs of protein side-chain and DNA or RNA phosphate groups. Using three protein–DNA complexes, we demonstrate that site-specific oxygen-to-sulfur substitution in phosphate groups allows for identification of NMR signals from the protein s...

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Pubblicato in:J Biomol NMR
Autori principali: Anderson, Kurtis M., Nguyen, Dan, Esadze, Alexandre, Zandarashvili, Levani, Gorenstein, David G., Iwahara, Junji
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4433575/
https://ncbi.nlm.nih.gov/pubmed/25690740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-9909-8
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