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Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes

RNA polymerase binds tightly to DNA to recognize promoters with high specificity but then releases these contacts during the initial stage of transcription. We report a site-specific crosslinking approach to map the DNA path in bacterial transcription intermediates at amino acid and nucleotide resol...

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Библиографические подробности
Опубликовано в: :Mol Cell
Главные авторы: Winkelman, Jared T., Winkelman, Bradford T., Boyce, Julian, Maloney, Michael F., Chen, Albert Y., Ross, Wilma, Gourse, Richard L.
Формат: Artigo
Язык:Inglês
Опубликовано: 2015
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4561013/
https://ncbi.nlm.nih.gov/pubmed/26257284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2015.06.037
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