ABORTIVE INITIATION AND PRODUCTIVE INITIATION BY RNA POLYMERASE INVOLVE DNA SCRUNCHING: Single-molecule-nanomanipulation experiments--in which magnetic tweezers are used to monitor RNA-polymerase-induced DNA unwinding with ~1 bp resolution and ~1 s temporal resolution--show that both abortive initiation and productive initiation by RNA polymerase involve DNA “scrunching.”

Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA “scrunching”--in which RNA polymerase (RNAP) remains stationary and unwinds and pulls into itself downstream DNA--that scrunching requires RNA synthesis, and that scrunching depends on RNA length. We show furth...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Revyakin, Andrey, Liu, Chenyu, Ebright, Richard H., Strick, Terence R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2754787/
https://ncbi.nlm.nih.gov/pubmed/17110577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1131398
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados