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ABORTIVE INITIATION AND PRODUCTIVE INITIATION BY RNA POLYMERASE INVOLVE DNA SCRUNCHING: Single-molecule-nanomanipulation experiments--in which magnetic tweezers are used to monitor RNA-polymerase-induced DNA unwinding with ~1 bp resolution and ~1 s temporal resolution--show that both abortive initiation and productive initiation by RNA polymerase involve DNA “scrunching.”

Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA “scrunching”--in which RNA polymerase (RNAP) remains stationary and unwinds and pulls into itself downstream DNA--that scrunching requires RNA synthesis, and that scrunching depends on RNA length. We show furth...

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Hlavní autoři: Revyakin, Andrey, Liu, Chenyu, Ebright, Richard H., Strick, Terence R.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2754787/
https://ncbi.nlm.nih.gov/pubmed/17110577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1131398
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