ABORTIVE INITIATION AND PRODUCTIVE INITIATION BY RNA POLYMERASE INVOLVE DNA SCRUNCHING: Single-molecule-nanomanipulation experiments--in which magnetic tweezers are used to monitor RNA-polymerase-induced DNA unwinding with ~1 bp resolution and ~1 s temporal resolution--show that both abortive initiation and productive initiation by RNA polymerase involve DNA “scrunching.”

Antzeko izenburuak

• Promoter unwinding and promoter clearance by RNA polymerase: Detection by single-molecule DNA nanomanipulation
  nork: Revyakin, Andrey, et al.
  Argitaratua: (2004)
• Nanomanipulation of Single RNA Molecules by Optical Tweezers
  nork: Stephenson, William, et al.
  Argitaratua: (2014)
• INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM: Single-molecule fluorescence-resonance-energy-transfer experiments establish that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center.
  nork: Kapanidis, Achillefs N., et al.
  Argitaratua: (2006)
• Transcription Initiation in a Single-subunit RNA Polymerase proceeds through DNA Scrunching and Rotation of the N-terminal subdomains
  nork: Tang, Guo-Qing, et al.
  Argitaratua: (2008)
• Transcription initiation by human RNA polymerase II visualized at single-molecule resolution
  nork: Revyakin, Andrey, et al.
  Argitaratua: (2012)