INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM: Single-molecule fluorescence-resonance-energy-transfer experiments establish that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center.

Ítems similars

• Transcription Initiation in a Single-subunit RNA Polymerase proceeds through DNA Scrunching and Rotation of the N-terminal subdomains
  per: Tang, Guo-Qing, et al.
  Publicat: (2008)
• ABORTIVE INITIATION AND PRODUCTIVE INITIATION BY RNA POLYMERASE INVOLVE DNA SCRUNCHING: Single-molecule-nanomanipulation experiments--in which magnetic tweezers are used to monitor RNA-polymerase-induced DNA unwinding with ~1 bp resolution and ~1 s temporal resolution--show that both abortive initiation and productive initiation by RNA polymerase involve DNA “scrunching.”
  per: Revyakin, Andrey, et al.
  Publicat: (2006)
• New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB
  per: Lagrange, Thierry, et al.
  Publicat: (1998)
• OPENING AND CLOSING OF THE BACTERIAL RNA POLYMERASE CLAMP: Single-molecule fluorescence experiments define RNA polymerase clamp conformation in transcription initiation and elongation
  per: Chakraborty, Anirban, et al.
  Publicat: (2012)
• RNA Polymerase Pausing during Initial Transcription
  per: Duchi, Diego, et al.
  Publicat: (2016)