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OPENING AND CLOSING OF THE BACTERIAL RNA POLYMERASE CLAMP: Single-molecule fluorescence experiments define RNA polymerase clamp conformation in transcription initiation and elongation

Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp formation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation, but cl...

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Detalhes bibliográficos
Main Authors: Chakraborty, Anirban, Wang, Dongye, Ebright, Yon W., Korlann, You, Kortkhonjia, Ekaterine, Chowdhury, Saikat, Wigneshweraraj, Sivaramesh, Irschik, Herbert, Jansen, Rolf, Nixon, B. Tracy, Knight, Jennifer, Weiss, Shimon, Ebright, Richard H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3626110/
https://ncbi.nlm.nih.gov/pubmed/22859489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1218716
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