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Promoter unwinding and promoter clearance by RNA polymerase: Detection by single-molecule DNA nanomanipulation

By monitoring the end-to-end extension of a mechanically stretched, supercoiled, single DNA molecule, we have been able directly to observe the change in extension associated with unwinding of approximately one turn of promoter DNA by RNA polymerase (RNAP). By performing parallel experiments with ne...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Revyakin, Andrey, Ebright, Richard H., Strick, Terence R.
פורמט: Artigo
שפה:Inglês
יצא לאור: National Academy of Sciences 2004
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC387324/
https://ncbi.nlm.nih.gov/pubmed/15037753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0307241101
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