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Promoter unwinding and promoter clearance by RNA polymerase: Detection by single-molecule DNA nanomanipulation
By monitoring the end-to-end extension of a mechanically stretched, supercoiled, single DNA molecule, we have been able directly to observe the change in extension associated with unwinding of approximately one turn of promoter DNA by RNA polymerase (RNAP). By performing parallel experiments with ne...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC387324/ https://ncbi.nlm.nih.gov/pubmed/15037753 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0307241101 |
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