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Structural Basis for Substrate and Oxygen Activation in Homoprotocatechuate 2,3-Dioxygenase: Roles of Conserved Active Site Histidine-200

Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the s...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Kovaleva, Elena G., Rogers, Melanie S., Lipscomb, John D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4558303/
https://ncbi.nlm.nih.gov/pubmed/26267790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00709
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