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3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Intra-thymic T cell development requires an intricate three-dimensional meshwork composed of various stromal cells, i.e., non-T cells. Thymocytes traverse this scaffold in a highly coordinated temporal and spatial order while sequentially passing obligatory check points, i.e., T cell lineage commitm...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Pinto, Sheena, Stark, Hans-Jürgen, Martin, Iris, Boukamp, Petra, Kyewski, Bruno
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4545168/
https://ncbi.nlm.nih.gov/pubmed/26275017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52614
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