Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs

Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-guide RNA (sgRNA) to generate site-specific DNA breaks. However, the uncertainties caused by...

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Udgivet i:Sci Rep
Main Authors: Wang, Xianlong, Zhou, Jinwei, Cao, Chunwei, Huang, Jiaojiao, Hai, Tang, Wang, Yanfang, Zheng, Qiantao, Zhang, Hongyong, Qin, Guosong, Miao, Xiangnan, Wang, Hongmei, Cao, Suizhong, Zhou, Qi, Zhao, Jianguo
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4543986/
https://ncbi.nlm.nih.gov/pubmed/26293209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13348
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