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A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl(-) Channels and Transporters
The last 15 years have been characterized by an explosion in the ability to overexpress and purify membrane proteins from prokaryotic organisms as well as from eukaryotes. This increase has been largely driven by the successful push to obtain structural information on membrane proteins. However, the...
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| Udgivet i: | J Vis Exp |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4541587/ https://ncbi.nlm.nih.gov/pubmed/25938223 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52369 |
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