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A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl(-) Channels and Transporters

The last 15 years have been characterized by an explosion in the ability to overexpress and purify membrane proteins from prokaryotic organisms as well as from eukaryotes. This increase has been largely driven by the successful push to obtain structural information on membrane proteins. However, the...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Basilio, Daniel, Accardi, Alessio
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2015
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4541587/
https://ncbi.nlm.nih.gov/pubmed/25938223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52369
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