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Measurement of Separase Proteolytic Activity in Single Living Cells by a Fluorogenic Flow Cytometry Assay

ESPL1/Separase, an endopeptidase, is required for centrosome duplication and separation of sister-chromatides in anaphase of mitosis. Overexpression and deregulated proteolytic activity of Separase as frequently observed in human cancers is associated with the occurrence of supernumerary centrosomes...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Haaß, Wiltrud, Kleiner, Helga, Müller, Martin C., Hofmann, Wolf-Karsten, Fabarius, Alice, Seifarth, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4534294/
https://ncbi.nlm.nih.gov/pubmed/26267133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0133769
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