Caricamento...
Fluorogenic Assay for Inhibitors of HIV-1 Protease with Sub-picomolar Affinity
A fluorogenic substrate for HIV-1 protease was designed and used as the basis for a hypersensitive assay. The substrate exhibits a k(cat) of 7.4 s(−1), K(M) of 15 μM, and an increase in fluorescence intensity of 104-fold upon cleavage, thus providing sensitivity that is unmatched in a continuous ass...
Salvato in:
| Pubblicato in: | Sci Rep |
|---|---|
| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group
2015
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4531283/ https://ncbi.nlm.nih.gov/pubmed/26261098 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11286 |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !
|