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Fluorogenic Assay for Inhibitors of HIV-1 Protease with Sub-picomolar Affinity
A fluorogenic substrate for HIV-1 protease was designed and used as the basis for a hypersensitive assay. The substrate exhibits a k(cat) of 7.4 s(−1), K(M) of 15 μM, and an increase in fluorescence intensity of 104-fold upon cleavage, thus providing sensitivity that is unmatched in a continuous ass...
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Publicado no: | Sci Rep |
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Main Authors: | , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Nature Publishing Group
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4531283/ https://ncbi.nlm.nih.gov/pubmed/26261098 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11286 |
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