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Fluorogenic Assay for Inhibitors of HIV-1 Protease with Sub-picomolar Affinity

A fluorogenic substrate for HIV-1 protease was designed and used as the basis for a hypersensitive assay. The substrate exhibits a k(cat) of 7.4 s(−1), K(M) of 15 μM, and an increase in fluorescence intensity of 104-fold upon cleavage, thus providing sensitivity that is unmatched in a continuous ass...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Windsor, Ian W., Raines, Ronald T.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4531283/
https://ncbi.nlm.nih.gov/pubmed/26261098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11286
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