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Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells

Haloalkane dehalogenase (HD) catalyzes the hydrolysis of haloalkanes via a covalent enzyme–substrate intermediate. Fusing a target protein to an HD variant that cannot hydrolyze the intermediate enables labeling of the target protein with a haloalkane in cellulo. The utility of extant probes is hamp...

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Bibliografische gegevens
Hoofdauteurs: Watkins, Rex W., Lavis, Luke D., Kung, Vanessa M., Los, Georgyi V., Raines, Ronald T.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2009
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2800956/
https://ncbi.nlm.nih.gov/pubmed/19763299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b907664f
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