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Fluorogenic Label to Quantify the Cytosolic Delivery of Macromolecules
The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coli β-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GL...
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| Glavni autori: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2013
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3588161/ https://ncbi.nlm.nih.gov/pubmed/23340874 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3mb25552b |
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