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A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells
A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a “trimethyl lock” that is triggered by cleavage of an ether bond. In vitro, fluorescence was manifested by the CYP1A1 isozyme with k(cat)/K(M) = 8.8 × 10(3) M(−1)...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2586036/ https://ncbi.nlm.nih.gov/pubmed/18595692 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2008.06.015 |
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