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Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Haloalkane dehalogenase (HD) catalyzes the hydrolysis of haloalkanes via a covalent enzyme–substrate intermediate. Fusing a target protein to an HD variant that cannot hydrolyze the intermediate enables labeling of the target protein with a haloalkane in cellulo. The utility of extant probes is hamp...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2800956/ https://ncbi.nlm.nih.gov/pubmed/19763299 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b907664f |
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