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Establishment and Validation of a Non-Radioactive Method for In Vitro Transcription Assay Using Primer Extension and Quantitative Real Time PCR

Primer extension-dependent in vitro transcription assay is one of the most important approaches in the research field of gene transcription. However, conventional in vitro transcription assays incorporates radioactive isotopes that cause environmental and health concerns and restricts its scope of a...

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Detalles Bibliográficos
Publicado en:PLoS One
Main Authors: Wang, Juan, Zhao, Shasha, Zhou, Ying, Wei, Yun, Deng, Wensheng
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4529316/
https://ncbi.nlm.nih.gov/pubmed/26252791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0135317
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