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Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs
There has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple, robust, inexpensive assay for quantitative analysis of microRNAs and short-interfering RNAs. The method relies on primer extension conversion of RNA to cDNA by reverse transcr...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Copyright 2005 by RNA Society
2005
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370860/ https://ncbi.nlm.nih.gov/pubmed/16244135 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2148705 |
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