Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs

There has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple, robust, inexpensive assay for quantitative analysis of microRNAs and short-interfering RNAs. The method relies on primer extension conversion of RNA to cDNA by reverse transcr...

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Main Authors: RAYMOND, CHRISTOPHER K., ROBERTS, BRIAN S., GARRETT-ENGELE, PHILLIP, LIM, LEE P., JOHNSON, JASON M.
Formato: Artigo
Idioma:Inglês
Publicado em: Copyright 2005 by RNA Society 2005
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Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370860/
https://ncbi.nlm.nih.gov/pubmed/16244135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2148705
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