Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells

Hepatic stellate cells (HSC) are the main effector cells for liver fibrosis. We aimed at optimizing HSC isolation by an additional step of fluorescence-activated cell sorting (FACS) via a UV laser. HSC were isolated from livers of healthy mice and animals subjected to experimental fibrosis. HSC isol...

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Detalhes bibliográficos
Publicado no:Anal Cell Pathol (Amst)
Main Authors: Bartneck, Matthias, Warzecha, Klaudia Theresa, Tag, Carmen Gabriele, Sauer-Lehnen, Sibille, Heymann, Felix, Trautwein, Christian, Weiskirchen, Ralf, Tacke, Frank
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4519541/
https://ncbi.nlm.nih.gov/pubmed/26258009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/417023
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