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Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells

Hepatic stellate cells (HSC) are the main effector cells for liver fibrosis. We aimed at optimizing HSC isolation by an additional step of fluorescence-activated cell sorting (FACS) via a UV laser. HSC were isolated from livers of healthy mice and animals subjected to experimental fibrosis. HSC isol...

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Détails bibliographiques
Publié dans:Anal Cell Pathol (Amst)
Auteurs principaux: Bartneck, Matthias, Warzecha, Klaudia Theresa, Tag, Carmen Gabriele, Sauer-Lehnen, Sibille, Heymann, Felix, Trautwein, Christian, Weiskirchen, Ralf, Tacke, Frank
Format: Artigo
Langue:Inglês
Publié: Hindawi Publishing Corporation 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4519541/
https://ncbi.nlm.nih.gov/pubmed/26258009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/417023
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