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Small-Molecule High-Throughput Screening Utilizing Xenopus Egg Extract

Screens for small-molecule modulators of biological pathways typically utilize cultured cell lines, purified proteins, or, recently, model organisms (e.g., zebrafish, Drosophila, C. elegans). Herein, we describe a method for using Xenopus laevis egg extract, a biologically active and highly tractabl...

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Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Broadus, Matthew R., Yew, P. Renee, Hann, Stephen R., Lee, Ethan
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4492114/
https://ncbi.nlm.nih.gov/pubmed/25618336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2269-7_5
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