Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to γ-cyclodextrin (CD(8)). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD(8) and a broad pH activity r...

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Bibliografische gegevens
Gepubliceerd in:FEBS Open Bio
Hoofdauteurs: Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2015
Onderwerpen:
Research article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491590/
https://ncbi.nlm.nih.gov/pubmed/26155461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2015.06.002
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