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Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to γ-cyclodextrin (CD(8)). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD(8) and a broad pH activity r...

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Bibliografiske detaljer
Udgivet i:FEBS Open Bio
Main Authors: Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491590/
https://ncbi.nlm.nih.gov/pubmed/26155461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2015.06.002
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