Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to γ-cyclodextrin (CD(8)). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD(8) and a broad pH activity r...

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Dettagli Bibliografici
Pubblicato in:FEBS Open Bio
Autori principali: Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2015
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Research article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491590/
https://ncbi.nlm.nih.gov/pubmed/26155461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2015.06.002
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