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Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9
RATIONALE: The participation of endothelial cells (EC) in many physiological and pathological processes is widely modeled using human EC cultures, but genetic manipulation of these untransformed cells has been technically challenging. Clustered regularly interspaced short palindromic repeats (CRISPR...
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| Publicado no: | Circ Res |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4490936/ https://ncbi.nlm.nih.gov/pubmed/25940550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/CIRCRESAHA.117.306290 |
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