Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9

RATIONALE: The participation of endothelial cells (EC) in many physiological and pathological processes is widely modeled using human EC cultures, but genetic manipulation of these untransformed cells has been technically challenging. Clustered regularly interspaced short palindromic repeats (CRISPR...

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Vydáno v:Circ Res
Hlavní autoři: Abrahimi, Parwiz, Chang, William G., Kluger, Martin S., Qyang, Yibing, Tellides, George, Saltzman, W. Mark, Pober, Jordan S.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4490936/
https://ncbi.nlm.nih.gov/pubmed/25940550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/CIRCRESAHA.117.306290
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