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Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9
RATIONALE: The participation of endothelial cells (EC) in many physiological and pathological processes is widely modeled using human EC cultures, but genetic manipulation of these untransformed cells has been technically challenging. Clustered regularly interspaced short palindromic repeats (CRISPR...
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| Publié dans: | Circ Res |
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| Auteurs principaux: | , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4490936/ https://ncbi.nlm.nih.gov/pubmed/25940550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/CIRCRESAHA.117.306290 |
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