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Efficient CRISPR/Cas9-Mediated Mutagenesis in Primary Murine T Lymphocytes: CRISPR-mediated gene-disruption in murine T cells

The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling and function. Manipulation of T cell clones and primary T cells has been accomplished primarily through overexpression or gene-silencing studies using cDNAs or shRN...

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Detalhes bibliográficos
Publicado no:Curr Protoc Immunol
Main Authors: Huang, Bonnie, Haurum Johansen, Kristoffer, Schwartzberg, Pamela L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6340735/
https://ncbi.nlm.nih.gov/pubmed/30312021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.62
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