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Efficient CRISPR/Cas9-Mediated Mutagenesis in Primary Murine T Lymphocytes: CRISPR-mediated gene-disruption in murine T cells
The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling and function. Manipulation of T cell clones and primary T cells has been accomplished primarily through overexpression or gene-silencing studies using cDNAs or shRN...
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| 發表在: | Curr Protoc Immunol |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2018
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6340735/ https://ncbi.nlm.nih.gov/pubmed/30312021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.62 |
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