Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures

BACKGROUND: CRISPR/Cas9 system is a powerful method to investigate the role of genes by introducing a mutation selectively and efficiently to specific genome positions in cell and animal lines. However, in primary neuron cultures, this method is affected by the issue that the effectiveness of CRISPR...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:BMC Neurosci
Prif Awduron: Tsunematsu, Hiroto, Uyeda, Akiko, Yamamoto, Nobuhiko, Sugo, Noriyuki
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2017
Pynciau:
Methodology Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5540436/
https://ncbi.nlm.nih.gov/pubmed/28764650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12868-017-0377-9
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