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Live cell imaging of duplex siRNA intracellular trafficking

Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Hirsch, Markus, Helm, Mark
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2015
Teme:
RNA
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4482072/
https://ncbi.nlm.nih.gov/pubmed/25870407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv307
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