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Live cell imaging of duplex siRNA intracellular trafficking

Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Hirsch, Markus, Helm, Mark
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2015
Fag:
RNA
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4482072/
https://ncbi.nlm.nih.gov/pubmed/25870407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv307
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