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Live cell imaging of duplex siRNA intracellular trafficking

Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Hirsch, Markus, Helm, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
RNA
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4482072/
https://ncbi.nlm.nih.gov/pubmed/25870407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv307
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