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Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides

BACKGROUND: The 5′-triphosphorylated, 2′-5′-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading viral and cellular RNA leading to inhibition of viral replication. The 2-5A syste...

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Publicat a:BMC Biochem
Autors principals: Poulsen, Jesper Buchhave, Kjær, Karina Hansen, Justesen, Just, Martensen, Pia Møller
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4481073/
https://ncbi.nlm.nih.gov/pubmed/26113370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12858-015-0043-8
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