Use of Robotized DNA Isolation and Real-Time PCR To Quantify and Identify Close Correlation between Levels of Neisseria meningitidis DNA and Lipopolysaccharides in Plasma and Cerebrospinal Fluid from Patients with Systemic Meningococcal Disease

The present study, using robotized DNA isolation and quantitative PCR based on the Neisseria meningitidis-specific capsular transport A gene, demonstrates the ease, rapidity, specificity, and sensitivity of quantifying neisserial DNA in plasma (n = 65) and cerebrospinal fluid (CSF) (n = 12) from pat...

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Bibliographic Details
Main Authors: Øvstebø, Reidun, Brandtzaeg, Petter, Brusletto, Berit, Haug, Kari Bente Foss, Lande, Knut, Høiby, Ernst Arne, Kierulf, Peter
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC446236/
https://ncbi.nlm.nih.gov/pubmed/15243048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.7.2980-2987.2004
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