Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with micro...

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Main Authors: Teira, Eva, Reinthaler, Thomas, Pernthaler, Annelie, Pernthaler, Jakob, Herndl, Gerhard J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2004
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Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC444763/
https://ncbi.nlm.nih.gov/pubmed/15240332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4411-4414.2004
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