Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with micro...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Teira, Eva, Reinthaler, Thomas, Pernthaler, Annelie, Pernthaler, Jakob, Herndl, Gerhard J.
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2004
Subjects:	Methods
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC444763/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15240332/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.7.4411-4414.2004
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