Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450(BM3) for Selective Hydroxylation of Large Substrates

Combinatorial alanine substitution of active site residues in a thermostable cytochrome P450(BM3) (BM3) variant was used to generate BM3 variants with activity on large substrates. Selective hydroxylation of methoxymethylated monosaccharides, alkaloids, and steroids was thus made possible. This appr...

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Vydáno v:Chembiochem
Hlavní autoři: Lewis, Jared C., Mantovani, Simone M., Fu, Yu, Snow, Christopher D., Komor, Russell S., Wong, Chi-Huey, Arnold, Frances H.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4447097/
https://ncbi.nlm.nih.gov/pubmed/21108271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201000565
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