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Enantioselective α-Hydroxylation of 2-Arylacetic Acid Derivatives and Buspirone Catalyzed by Engineered Cytochrome P450 BM-3

[Image: see text] Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A1 subfamily) efficiently catalyzes the α-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.

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Bibliografski detalji
Glavni autori: Landwehr, Marco, Hochrein, Lisa, Otey, Christopher R., Kasrayan, Alex, Bäckvall, Jan-E., Arnold, Frances H.
Format: Artigo
Jezik:Inglês
Izdano: 2006
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2551755/
https://ncbi.nlm.nih.gov/pubmed/16669674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja061261x
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