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Kinetic characterization of intramolecular and intermolecular hammerhead RNAs with stem II deletions.

A method is described to obtain intramolecular cleavage rates for the hammerhead ribozyme during in vitro transcription. By avoiding RNA purification and renaturation, the potential for formation of inactive conformations of the RNA is minimized. By showing that an intramolecular hammerhead and a cl...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
I whakaputaina i:Proc Natl Acad Sci U S A
Ngā kaituhi matua: Long, D M, Uhlenbeck, O C
Hōputu: Artigo
Reo:Inglês
I whakaputaina: National Academy of Sciences 1994
Ngā marau:
Urunga tuihono:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44321/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7518924/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.15.6977
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