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Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases.

We have synthesized 13 hammerhead ribozyme variants, each containing an abasic residue at a specific position of the catalytic core. The activity of each of the variants is significantly reduced. In four cases, however, activity can be rescued by exogenous addition of the missing base. For one varia...

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Detalhes bibliográficos
Main Authors: Peracchi, A, Beigelman, L, Usman, N, Herschlag, D
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC38090/
https://ncbi.nlm.nih.gov/pubmed/8876168
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