Kinetic characterization of intramolecular and intermolecular hammerhead RNAs with stem II deletions.
A method is described to obtain intramolecular cleavage rates for the hammerhead ribozyme during in vitro transcription. By avoiding RNA purification and renaturation, the potential for formation of inactive conformations of the RNA is minimized. By showing that an intramolecular hammerhead and a cl...
Guardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1994
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44321/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7518924/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.15.6977 |
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