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Library preparation for highly accurate population sequencing of RNA viruses

Circular resequencing (CirSeq) is a novel technique for efficient and highly accurate next-generation sequencing (NGS) of RNA virus populations. The foundation of this approach is the circularization of fragmented viral RNAs, which are then redundantly encoded into tandem repeats by ‘rolling-circle’...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Acevedo, Ashley, Andino, Raul
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4418788/
https://ncbi.nlm.nih.gov/pubmed/24967624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.118
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