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Evaluating bias-reducing protocols for RNA sequencing library preparation

BACKGROUND: Next-generation sequencing does not yield fully unbiased estimates for read abundance, which may impact on the conclusions that can be drawn from sequencing data. The ligation step in RNA sequencing library generation is a known source of bias, motivating developments in enzyme technolog...

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Detalhes bibliográficos
Main Authors: Jackson, Thomas J, Spriggs, Ruth V, Burgoyne, Nicholas J, Jones, Carolyn, Willis, Anne E
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4117970/
https://ncbi.nlm.nih.gov/pubmed/25001197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-15-569
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