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Measuring the activity of protein variants on a large scale using deep mutational scanning
Deep mutational scanning marries selection for protein function to high-throughput DNA sequencing in order to quantify the activity of variants of a protein on a massive scale. First, an appropriate selection system for the protein function of interest is identified and validated. Second, a library...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4412028/ https://ncbi.nlm.nih.gov/pubmed/25167058 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.153 |
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