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Identification of Destabilizing and Stabilizing Mutations of Ste2p, a G Protein Coupled Receptor in Saccharomyces cerevisiae

The isolation of mutations affecting the stabilities of transmembrane proteins is useful for enhancing the suitability of proteins for structural characterization and for identification of determinants of membrane protein stability. We have pursued a strategy for identification of stabilized variant...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Zuber, Jeffrey, Danial, Shairy Azmy, Connelly, Sara M., Naider, Fred, Dumont, Mark E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4405178/
https://ncbi.nlm.nih.gov/pubmed/25647246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi501314t
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