Differential Interactions of Fluorescent Agonists and Antagonists with the Yeast G Protein Coupled Receptor Ste2p

We describe a rapid method to probe for mutations in cell-surface ligand-binding proteins that affect the environment of bound ligand. The method uses fluorescence activated cell sorting to screen randomly-mutated receptors for substitutions that alter the fluorescence emission spectrum of environme...

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Detalhes bibliográficos
Main Authors: Mathew, Elizabeth, Bajaj, Anshika, Connelly, Sara M., Sargsyan, Hasmik, Ding, Fa-Xiang, Hajduczok, Alexander G., Naider, Fred, Dumont, Mark E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3104124/
https://ncbi.nlm.nih.gov/pubmed/21477594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.03.059
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