Affinity Purification and Characterization of a G-Protein Coupled Receptor, Saccharomyces cerevisiae Ste2p

We present an example of expression and purification of a biologically active G protein coupled receptor (GPCR) from yeast. An expression vector was constructed to encode the Saccharomyces cerevisiae GPCR α-factor receptor (Ste2p, the STE2 gene product) containing a 9-amino acid sequence of rhodopsi...

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Bibliographic Details
Main Authors: Lee, Byung-Kwon, Jung, Kyung-Sik, Son, Cagdas, Kim, Heejung, VerBerkmoes, Nathan C., Arshava, Boris, Naider, Fred, Becker, Jeffrey M.
Format: Artigo
Language:Inglês
Published: 2007
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2065862/
https://ncbi.nlm.nih.gov/pubmed/17646109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.06.002
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