A Method for Enzymic Extraction and the Measurement of Chloroplast RNA
A method is described for measuring RNA associated with chloroplast thylakoid membranes. Washed thylakoids are incubated with ribonucleases A and T(1), under low Mg(2+) conditions, to release hydrolyzed RNA into solution. After removing the membranes by centrifugation, the mono- and oligonucleotides...
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| Publicado no: | Plant Physiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440415/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661271/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.65.4.746 |
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