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A Method for Enzymic Extraction and the Measurement of Chloroplast RNA

A method is described for measuring RNA associated with chloroplast thylakoid membranes. Washed thylakoids are incubated with ribonucleases A and T(1), under low Mg(2+) conditions, to release hydrolyzed RNA into solution. After removing the membranes by centrifugation, the mono- and oligonucleotides...

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Bibliografiske detaljer
Udgivet i:Plant Physiol
Principais autores: Fish, Leonard, Jagendorf, André T.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1980
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440415/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661271/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.65.4.746
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